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    ژنتیک مولکولی

    مارتین لوتر هاله ویتنبرگ ، هاله، المان

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  • تولید لاین های دابل هاپلویید در گیاهان با ارزش اقتصادی با استفاده از ابزارهای مولکولی
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    Generation of haploid plants

    A Houben, R Karimi-Ashiyani, T Ishii, N Stein, J Kumlehm, C Bolduan, ...
    Journal Papers , , {Pages }

    Abstract

    Centromere engineering as an emerging tool for haploid plant production: Advances and challenges

    R Karimi-Ashtiyani
    Journal Papers , , {Pages }

    Abstract

    Generation of haploid plants

    Journal Papers , 2017 October 5, {Pages }

    Abstract

    Point Mutation of CENH3 (CENP-A) Impairs Loading to Centromeres and Induces Haploid Plants

    T Ishii, R Karimi-Ashtiyani, M Niessen, N Stein, S Heckmann, M Gurushidze, AM Banaei-Moghaddam, J Fuchs, V Schubert, K Koch, O Weiss, D Demidov, K Schmidt, J Kumlehn, A Houben
    Conference PapersCYTOGENETIC AND GENOME RESEARCH , Volume 148 , Issue 03-Feb, 2016 January 1, {Pages 143-144 }

    Abstract

    Haploidization via chromosome elimination: means and mechanisms

    Takayoshi Ishii, Raheleh Karimi-Ashtiyani, Andreas Houben
    Journal Papers , Volume 67 , 2016 April 29, {Pages 421-438 }

    Abstract

    The ability to generate haploids and subsequently induce chromosome doubling significantly accelerates the crop breeding process. Haploids have been induced through the generation of plants from haploid tissues (in situ gynogenesis and androgenesis) and through the selective loss of a parental chromosome set via inter- or intraspecific hybridization. Here, we focus on the mechanisms responsible for this selective chromosome elimination. CENH3, a variant of the centromere-specific histone H3, has been exploited to create an efficient method of haploid induction, and we discuss this approach in some detail. Parallels have been drawn with chromosome-specific elimination, which occurs as a normal part of differentiation and sex determination in

    Point mutation impairs centromeric CENH3 loading and induces haploid plants

    Raheleh Karimi-Ashtiyani, Takayoshi Ishii, Markus Niessen, Nils Stein, Stefan Heckmann, Maia Gurushidze, Ali Mohammad Banaei-Moghaddam, J?rg Fuchs, Veit Schubert, Kerstin Koch, Oda Weiss, Dmitri Demidov, Klaus Schmidt, Jochen Kumlehn, Andreas Houben
    Journal PapersProceedings of the National Academy of Sciences , Volume 112 , Issue 36, 2015 September 8, {Pages 11211-11216 }

    Abstract

    The chromosomal position of the centromere-specific histone H3 variant CENH3 (also called “CENP-A”) is the assembly site for the kinetochore complex of active centromeres. Any error in transcription, translation, modification, or incorporation can affect the ability to assemble intact CENH3 chromatin and can cause centromere inactivation [Allshire RC, Karpen GH (2008) Nat Rev Genet 9 (12): 923–937]. Here we show that a single-point amino acid exchange in the centromere-targeting domain of CENH3 leads to reduced centromere loading of CENH3 in barley, sugar beet, and Arabidopsis thaliana. Haploids were obtained after cenh3 L130F-complemented cenh3-null mutant plants were crossed with wild-type A. thaliana. In contrast, in a noncompeting

    The differential loading of two barley CENH3 variants into distinct centromeric substructures is cell type-and development-specific

    Takayoshi Ishii, Raheleh Karimi-Ashtiyani, Ali Mohammad Banaei-Moghaddam, Veit Schubert, J?rg Fuchs, Andreas Houben
    Journal PapersChromosome Research , Volume 23 , Issue 2, 2015 June 1, {Pages 277-284 }

    Abstract

    The organization of centromeric chromatin of diploid barley (Hordeum vulgare) encoding two (α and β) CENH3 variants was analysed by super-resolution microscopy. Antibody staining revealed that both CENH3 variants are organized in distinct but intermingled subdomains in interphase, mitotic and meiotic centromeres. Artificially extended chromatin fibres illustrate that these subdomains are formed by polynucleosome clusters. Thus, a CENH3 variant-specific loading followed by the arrangement into specific intermingling subdomains forming the centromere region appears. The CENH3 composition and transcription vary among different tissues. In young embryos, most interphase centromeres are composed of both CENH3 variants, while in

    Organization, Dynamics and Modification of Chromatin in Plants

    Andreas Houben, Tom?s Naranjo, Mar?a J Puertas
    Journal PapersCytogenet Genome Res , Volume 143 , Issue 4, 2014 January , {Pages 221-288 }

    Abstract

    Editors: Houben, A.(Gatersleben); Naranjo, T.; Puertas, MJ (Madrid)220 p., 88 fig, 67 in color, 14 tab., 2014 CHF 56.00/EUR 47.00/USD 66.00 (soft cover) CHF 67.00/EUR 56.00/USD 79.00 (online) Online version for institutional purchase, prices subject to change EUR price for Germany, USD price for USA and Latin America only

    Anti-phosphorylated histone H2AThr120: a universal microscopic marker for centromeric chromatin of mono-and holocentric plant species

    D Demidov, V Schubert, K Kumke, O Weiss, R Karimi-Ashtiyani, J Buttlar, S Heckmann, G Wanner, Q Dong, F Han, A Houben
    Journal PapersCytogenetic and genome research , Volume 143 , Issue 03-Jan, 2014 January , {Pages 150-156 }

    Abstract

    Based on the analysis of 20 different monocot and eudicot species, we propose that the centromeric distribution of the phosphorylated histone H2AThr120 is evolutionary highly conserved across species with mono- and holocentric chromosomes. Therefore, antibodies recognizing the phosphorylated threonine 120 of the histone H2A can serve as a universal marker for the cytological detection of centromeres of mono- and holokinetic plant species. In addition, super resolution microscopy of signals specific to the centromere-specific histone H3 variant CENH3 and to H2AThr120ph revealed that these histone variants are incorporated into different nucleosomes, which form distinct, partly intermingled chromatin domains. This specific arrangement of both

    Author Index Vol. 143, No. 1-3, 2014

    Magda Soukupova, Eva Nevrtalova, Jana Č?žkov?, Ivan Vogel, Radim Cegan, Roman Hobza, Boris Vyskot, Wenli Zhang, Tao Zhang, Yufeng Wu, Jiming Jiang, Stefania Del Prete, Javier Arp?n, Kaori Sakai, Philippe Andrey, Val?rie Gaudin, Shihan Yan, Qi Zhang, Yingn
    Journal PapersCytogenetic and Genome Research , Volume 143 , Issue 03-Jan, 2014 January , {Pages 219-220 }

    Abstract

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    Front & Back Matter

    Magda Soukupova, Eva Nevrtalova, Jana Č?žkov?, Ivan Vogel, Radim Cegan, Roman Hobza, Boris Vyskot, Wenli Zhang, Tao Zhang, Yufeng Wu, Jiming Jiang, Stefania Del Prete, Javier Arp?n, Kaori Sakai, Philippe Andrey, Val?rie Gaudin, Shihan Yan, Qi Zhang, Yingn
    Journal PapersCytogenetic and Genome Research , Volume 143 , Issue 03-Jan, 2014 January , {Pages }

    Abstract

    Editors: St?ck, M.(Berlin); Lamatsch, DK (Mondsee) 242 p., 49 fig., 31 in color, 10 tab., 2013 CHF 129.–/EUR 108.–/USD 152.00 (hard cover) CHF 155.–/EUR 130.–/USD 182.00 (online) Online version for institutional purchase Prices subject to change EUR price for Germany, USD price for USA only ISBN 978–3–318–02475–3 (hard cover) e-ISBN 978–3–318–02476–0

    Epigenetic control of cell division

    Andreas Houben, Dmitri Demidov, Raheleh Karimi-Ashtiyani
    Journal Papers , 2013 January , {Pages 155-175 }

    Abstract

    Posttranslational modification (PTM) of histone tails plays a critical role in the dynamic of chromatin and chromosomes. However, emerging evidence suggests that individual histone modifications do not reliably predict a single functional output [Sims and Reinberg (Nat Rev Mol Cell Biol 9:815–820, 2008)]. In plants, the cell cycle-dependent phosphorylation of histone H3 has been described best; it is hyperphosphorylated at serines 10/28 and at threonines 3/11/32 during both mitosis and meiosis in patterns that are specifically coordinated in both space and time. Although this posttranslational modification is highly conserved, data show that the chromosomal distribution of individual modifications can differ between groups of

    Formation and expression of pseudogenes on the B chromosome of rye

    Ali Mohammad Banaei-Moghaddam, Karla Meier, Raheleh Karimi-Ashtiyani, Andreas Houben
    Journal PapersThe Plant Cell , 2013 July 1, {Pages tpc. 113.111856 }

    Abstract

    B chromosomes (Bs) are dispensable components of the genomes of numerous species. In contrast with the prevalent view that Bs do not harbor genes, our recent sequence analysis revealed that Bs of rye (Secale cereale) are rich in gene-derived sequences. We compared these gene-like fragments of the rye B with their ancestral A-located counterparts and confirmed an A chromosomal origin and the pseudogenization of B-located gene-like fragments. About 15% of the pseudogene-like fragments on Bs are transcribed in a tissue-type and genotype-specific manner. In addition, B-located sequences can cause in trans down- or upregulation of A chromosome–encoded genic fragments. Phenotypes and effects associated with the presence of Bs might be explained

    In vitro phosphorylation of histone H3 at threonine 3 by Arabidopsis haspin is strongly influenced by posttranslational modifications of adjacent amino acids

    Raheleh Karimi-Ashtiyani, Andreas Houben
    Journal PapersMol Plant , Volume 6 , Issue 2, 2013 March 1, {Pages 574-576 }

    Abstract

    Page 1. 1 Letter to the Editor In vitro phosphorylation of histone H3 at threonine 3 by Arabidopsis Haspin is strongly influenced by post-translational modifications of adjacent amino acids Raheleh Karimi-Ashtiyani*, and Andreas Houben Leibniz Institute Plant Genet & Crop Plant Research, 06466 Gatersleben, Germany *To whom correspondence should be addressed E-mail: karimi@ipk-gatersleben.de fax +49 39482 5137; phone +49 39482 5477 Kew words: histone H3 phosphorylation, cross-talk, Arabidopsis, Haspin, histone H3T3, histone H3T6 Dear Editor, It is well established that combinatorial post-translational histone modifications regulate many aspects of chromatin dynamics. The evolutionary conserved kinase Haspin specifically phosphorylates histon

    AtHaspin phosphorylates histone H3 at threonine 3 during mitosis and contributes to embryonic patterning in Arabidopsis

    Raheleh Karimi Ashtiyani, Ali Mohammad Banaei Moghaddam, Veit Schubert, Twan Rutten, J?rg Fuchs, Dmitri Demidov, Frank R Blattner, Andreas Houben
    Journal PapersThe Plant Journal , Volume 68 , Issue 3, 2011 November , {Pages 443-454 }

    Abstract

    Post‐translational histone modifications regulate many aspects of chromosome activity. Threonine 3 of histone H3 is highly conserved, but the significance of its phosphorylation is unclear, and the identity of the corresponding kinase in plants is unknown. Therefore, we characterized the candidate kinase in Arabidopsis thaliana, called AtHaspin. Recombinant AtHaspin in vitro phosphorylates histone H3 at threonine 3. Reduction of H3 threonine 3 phosphorylation level and reduced chromatin condensation in interphase nuclei by AtHaspin RNAi supports the proposition that this kinase is involved in histone H3 phosphorylation in vivo in mitotic cells. In addition, we provide a developmental function for a Haspin kinase. At the whole plant level

    Additive inheritance of histone modifications in Arabidopsis thaliana intra‐specific hybrids

    Ali M Banaei Moghaddam, Francois Roudier, Michael Seifert, Caroline B?rard, Marie‐Laure M Magniette, Raheleh Karimi Ashtiyani, Andreas Houben, Vincent Colot, Michael F Mette
    Journal PapersThe Plant Journal , Volume 67 , Issue 4, 2011 August , {Pages 691-700 }

    Abstract

    Plant genomes are earmarked with defined patterns of chromatin marks. Little is known about the stability of these epigenomes when related, but distinct genomes are brought together by intra‐species hybridization. Arabidopsis thaliana accessions and their reciprocal hybrids were used as a model system to investigate the dynamics of histone modification patterns. The genome‐wide distribution of histone modifications H3K4me2 and H3K27me3 in the inbred parental accessions Col‐0, C24 and Cvi and their hybrid offspring was compared by chromatin immunoprecipitation in combination with genome tiling array hybridization. The analysis revealed that, in addition to DNA sequence polymorphisms, chromatin modification variations exist among acces

    AtHaspin, a Putative Histone H3-specific Kinase in Arabidopsis Thaliana?

    Raheleh Karimi Ashtiyani
    Journal Papers , 2009 January , {Pages }

    Abstract

    AtHaspin, a putative histone H3-specific kinase in Arabidopsis thaliana?

    Raheleh Karimi Ashtiyani
    Journal Papers , 2009 January , {Pages }

    Abstract

    Eine Zellzyklus-abh?ngige Phosphorylierung von Histon H3 an der Position Threonin 3 existiert in Pflanzen. Dieser Vorgang korreliert mit dem Vorgang der Chromosomenkondensation. Eine potentielle H3Thr3-spezifische Haspin-?hnliche Kinase (genannt AtHapin) wurde in A. thaliana identifiziert. AtHaspin-RNAi-Pflanzen sind durch eine reduzierte H3Thr3 Phosphorylierung gekennzeichnet. Die h?chste Genaktivit?t von AtHaspin wurde in Sprossmeristemen und Bl?tenknospen detektiert. Homozygote AtHaspin T-DNA Mutanten sind nicht lebensf?hig, was darauf hindeutet, dass AtHaspin essentiell f?r die Funktion der Pflanze ist. Eine ver?nderte Genaktivit?t (via RNAi oder ?berexpression) resultiert in pleiotrope Effekte, welche durch fehlerhafte Entwicklung der

    Aurora1 phosphorylation activity on histone H3 and its cross‐talk with other post‐translational histone modifications in Arabidopsis

    Dmitri Demidov, Susann Hesse, Annegret Tewes, Twan Rutten, J?rg Fuchs, Raheleh Karimi Ashtiyani, Sandro Lein, Andreas Fischer, Gunter Reuter, Andreas Houben
    Journal PapersThe Plant Journal , Volume 59 , Issue 2, 2009 July , {Pages 221-230 }

    Abstract

    The enzymological properties of AtAurora1, a kinase responsible for the cell cycle‐dependent phosphorylation of histone H3 at S10, and its cross‐talk with other post‐translational histone modifications, were determined. In vitro phosphorylation of H3S10 by AtAurora1 is strongly increased by K9 acetylation, and decreased by K14 acetylation and T11 phosphorylation. However, S10 phosphorylation activity is unaltered by mono‐, di‐ or trimethylation of K9. An interference of H3K9 dimethylation by SUVR4 occurs by a pre‐existing phosphorylation at S10. Hence, cross‐talk in plants exists between phosphorylation of H3S10 and methylation, acetylation or phosphorylation of neighbouring amino acid residues. AtAurora1 undergoes autophosph

    Distribution patterns of phosphorylated Thr 3 and Thr 32 of histone H3 in plant mitosis and meiosis

    Houben A. Caperta AD1, Rosa M, Delgado M, Karimi R, Demidov D, Viegas W
    Journal Paperscytogenetic and genome research , 2008 February , {Pages }

    Abstract

    Cell cycle dependent phosphorylation of conserved N-terminal tail residues of histone H3 has been described in both animal and plant cells. Through cytogenetic approaches using different plant species we show a detailed description of distribution patterns of phosphorylated histone H3 at either threonine 3 or threonine 32 in mitosis and meiosis. In meristematic cells of the large genome species Secale cereale, Vicia faba and Hordeum vulgare we have found that phosphorylation of both threonine residues begins in prophase, and dephosphorylation occurs in late anaphase. However, in the small genome species Arabidopsis thaliana dephosphorylation occurs at anaphase. In the first division of meiosis of species with large genomes phosphorylation o

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    دروس نیمسال جاری

    • كارشناسي ارشد
      ژنوميك ( واحد)
      دانشکده کشاورزی، گروه بيوتكنولوژي كشاورزي
    • كارشناسي ارشد
      مباحث نوين در بيوتكنولوژي ( واحد)

    دروس نیمسال قبل

    • كارشناسي ارشد
      ژنتيك ملكولي ( واحد)
      دانشکده کشاورزی، گروه بيوتكنولوژي كشاورزي
    • كارشناسي ارشد
      روش تحقيق ( واحد)
    • دكتري
      مكانيسم هاي ملكولي پاسخ به تنش ها ( واحد)
    • دكتري
      سيتوژنتيك پيشرفته ( واحد)
    • 1399
      قورچي بيگي, اميرمحمد
      مهندسي پروموتر ژن amyE در باسيلوس سوبتيليس با استفاده از سيستم كريسپر به منظور افزايش توليد آنزيم آلفا آميلاز
    • 1399
      نوري كوهاني, فاطمه
    • 1400
      روحاني, مريم سادات
    • 1398
      لطفي, رضا
      تعيين ساختار پروتئين CENH3 در گياه Arabidopsis thaliana به منظور درك سازوكار حذف انتخابي ژنوم ناشي از آن
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