En
  • دکتری (1364)

    بیوشیمی کلینیکی

    کینگزکالج، انگلستان

  • کارشناسی‌ارشد (1358)

    بیوشیمی کلینیکی

    نیوکاسل، انگلستان

  • بررسی مسیرهای سیگنالینگ رشد و آپوپتوز و نکروپتوزدر سرطان
  • بیولوژی و بیوشیمی مولکولی در سرطان
  • تحقیقات سرطان

از سال 1373 استاد دانشگاه تربیت مدرس بوده و زمینه تحقیقاتی اینجانب بیوشیمی بالینی، بیوشیمی و بیولوژی سرطان میباشد که در این زمینه مسیرهای رشد وانواع مرگهای سلولی (نکروز، آپوپتوز و نکروپتوز) در تومورهای بدخیم، خوش خیم و نرمال و همچنین در محیط کشت سلولهای سرطانی به منظور شناخت، تشخیص و درمان سرطان مورد بررسی قرار گرفته و ادامه دارد

ارتباط

رزومه

Expression of SR-B1 receptor in breast cancer cell lines, MDAMB-468 and MCF-7: Effect on cell proliferation and apoptosis

N Karimi, FSK Tehrani
Journal Papers , , {Pages }

Abstract

Addendum to: Expression of cGMP-dependent protein kinase, PKGIα, PKGIß, and PKGII in malignant and benign breast tumors

F Karami-Tehrani, F Fallahian, M Atri
Journal Papers , , {Pages }

Abstract

Evaluation of LDL Receptor and Scavenger Receptor, Class B, Type 1 in the malignant and benign breast tumors: The Correlation with the expression of miR-199a-5p, miR-199b-5p?…

Behzad Jamalzei, Fatemeh Soghra Karami Tehrani, Morteza Atri
Journal PapersGene , 2020 April 30, {Pages 144720 }

Abstract

AimsThe purpose of present study was to examine the correlations of LDL (LDLR) and HDL (SR-B1) receptors with lipoproteins, miR-199a-5p, miR-199b-5p, miR-455-5p in the malignant and benign breast tumors.MethodsTotal cholesterol-rich-lipoproteins and the receptors were determined using enzymatic-homogeneous and ELISA methods. The expression levels of miRNAs were detected by qRT-PCR.ResultsReceptor expressions and lipoproteins concentration were significantly higher in the malignant tumors (p<0.05). Positive correlation was found for LDLR with Ki67% and Her2+. HDL-C content of TNBC tumors was higher than those of Non-TNBC (p<0.05). The expression level of miR-199a-5p was found to be downregulated significantly in the malignant tumors of <2cm,

Differential expression of miR-1297, miR-3191-5p, miR-4435, and miR-4465 in malignant and benign breast tumors

Abbas Mosapour, Fatemeh Soghra Karami Tehrani, Morteza Atri
Journal PapersIranian Journal of Basic Medical Sciences , 2020 May 30, {Pages }

Abstract

Objective(s): MicroRNAs (miRs) are a class of small non-coding RNAs which are associated with tumor growth and progression. In the present study, we assessed the expression of selected miRs in malignant, benign, and adjacent normal breast tissues. Materials and Methods: The expression of miR-1297, miR-3191-5P, miR-4435, and miR-4465 were evaluated in malignant (n=50), benign (n=35), and adjacent normal breast tissues (n=20) using qRT-PCR. Receiver operating characteristic curves and the area under the ROC curve and AUC were generated for evaluating the diagnostic values of miRs. To evaluate diagnostic efficacy, miRs-based score was obtained using the logistic regression model. Results: Among malignant tumors, the expression of miR-1297, miR

Expression level of VLDL receptor and VLDL-c levels in the malignant and benign breast tumors: The correlation with miRNA-4465 and miRNA-1297

Abbas Mosapour, Fatemeh Soghra Karami Tehrani, Morteza Atri
Journal PapersMolecular and Cellular Probes , Volume 53 , 2020 October 1, {Pages 101624 }

Abstract

Breast cancer as one of the most prevalent cancers has high morbidity and mortality. Very low-density lipoprotein receptor (VLDLR) is a multifunctional receptor which plays a principal role in the tumor development through affecting cell metastasis and proliferation. The VLDLR as a target for miRNA-4465 and miRNA-1297 was predicted using bioinformatics analysis. Tissue specimens of malignant (n?=?50), benign (n?=?35) and corresponding normal breast (n?=?20) were considered to evaluate the expression of VLDLR using RT-qPCR and western blotting. The VLDL cholesterol (VLDL-C) levels were quantified using a colorimetric assay. The relative VLDLR expression was found in the malignant tumors, which was significantly lower than that in the normal

Evaluation of LDL receptor and Scavenger Receptor, Class B, Type 1 in the malignant and benign breast tumors: The correlation with the expression of miR-199a-5p, miR-199b-5p …

B Jamalzei, FSK Tehrani, M Atri
Journal Papers , , {Pages }

Abstract

Soluble guanylate cyclase isoenzymes: The expression of α1, α2, β1, and β2 subunits in the benign and malignant breast tumors

Milad Mohammadoo‐Khorasani, Fatemeh Karami Tehrani, Morteza Atri
Journal PapersJournal of cellular physiology , 2019 July 4, {Pages }

Abstract

Soluble guanylate cyclase (sGC) encompasses α and β subunits. This study examined the expression of α1, α2, β1, and β2 subunits in the malignant and benign breast tumors using the Western blot  analysis. Both benign and malignant tumors showed a significantly higher expression of the?α1 subunit in comparison with normal tissues (p < 0.0001). In contrast, the expression of α2 and β2 sGC were significantly lower in these tumors than normal tissues (p < .0015 and p < .001, p < .007 and p < .0001, respectively). The expression level of α1 sGC was significantly correlated with ER + PR+ (p < .0001). A significant correlation was also detected for sGC‐α1 and ‐α2 expression with c‐erbB2‐negative st

Differential expression of alternative transcripts of soluble guanylyl cyclase, GYCY1a3 and GUCY1b3 genes, in the malignant and benign breast tumors

Milad Mohammadoo khorasani, Fatemeh Karami Tehrani, Seyed Mohammad Reza Parizadeh, Morteza Atri
Journal PapersNitric Oxide , Volume 83 , 2019 February 1, {Pages 65-71 }

Abstract

Extensive alterations in splicing is one of the molecular indicator for human cancers. Soluble guanylyl cyclase (sGC), an obligatory heterodimer, is composed of α1 and β1 subunits. Each subunit is encoded by a separate gene, GUCY1a3 and GUCY1b3, correspondingly. sGC activity has been regulated by an alternative splicing and it has an important effect on the breast cancer. sGC alternative splicing has been evaluated in the 55 malignant, 25 benign and 30 normal breast tissues using qRT-PCR and RT-PCR. The differences between groups were analyzed by Mann-Whitney U. The expression of six different splice forms have been detected, three for α1 and three for β1 sGC. Expressions of Tr1, Tr2 β1 sGC and Tr7, Tr6 α1 sGC mRNA in the malignant br

Targeting cell necroptosis and apoptosis induced by Shikoning via receptor interacting protein kinases in estrogen receptor positive breast cancer cell line, MCF-7

Zahra Shahsavari, Fatemeh Karami Tehrani, Siamak Salami
Journal PapersAnti-Cancer Agents in Medicinal Chemistry , Volume 18 , Issue 2, 2018 January , {Pages 245-254 }

Abstract

Background: Recognition of a new therapeutic agent may activate an alternative programmed cell death for the treatment of breast cancer. Objective: Here, it has been tried to evaluate the effects of Shikonin, a naphthoquinone derivative of Lithospermum erythrorhizon, on the induction of necroptosis and apoptosis mediated by RIPK1-RIPK3 in the ER+ breast cancer cell line, MCF-7. Methods: In the current study, cell death modalities, cell cycle patterns, RIPK1 and RIPK3 expressions, caspase-3 and caspase-8 activities, reactive oxygen species and mitochondrial membrane potential have been evaluated in the Shikonin-treated MCF-7 cells. Results: Necroptosis and apoptosis have been occurred by Shikonin, with a significant increase in RIPK1 and RIP

Evaluation of RIP1K and RIP3K expressions in the malignant and benign breast tumors

Fatemeh Karami-Tehrani, Amin Rahimi Malek, Zahra Shahsavari, Morteza Atri
Journal PapersTumor Biology , Volume 37 , Issue 7, 2016 July , {Pages 8849–8856 }

Abstract

Receptor-interacting protein kinase 1 (RIP1K) and RIP3K belong to RIPK family, which regulate cell survival and cell death. In the present investigation, the expression levels of RIP1K and RIP3K were evaluated in the 30 malignant, 15 benign, and 20 normal breast tissues, and their correlation with clinicopathological characteristics was also studied. The expression levels of RIP1K and RIP3K were determined, by western blot analysis. The relative RIP1K expression was significantly higher in the malignant and benign tumors when compared to those of normal tissues (P < 0.0001 and P < 0.001, respectively). However, the expression level of RIP3K was significantly lower in the malignant tumors than those of normal and benig

RIP1K and RIP3K provoked by shikonin induce cell cycle arrest in the triple negative breast cancer cell line, MDA-MB-468: necroptosis as a desperate programmed suicide pathway

Zahra Shahsavari, Fatemeh Karami-Tehrani, Siamak Salami, Mehran Ghasemzadeh
Journal PapersTumor Biology , Volume 37 , Issue 4, 2016 January , {Pages 4479-4491 }

Abstract

Resistance to cell death and reprogramming of metabolism are important in neoplastic cells. Increased resistance to apoptosis and recurrence of tumors are the major roadblocks to effective treatment of triple negative breast cancer. It has been thought that execution of necroptosis involves ROS generation and mitochondrial dysfunction in malignant cells. In this study, the effect of shikonin, an active substance from the dried root of Lithospermum erythrorhizon, on the induction of necroptosis or apoptosis, via RIP1K-RIP3K expressions has been examined in the triple negative breast cancer cell line. The expression levels of RIP1K and RIP3K, caspase-3 and caspase-8 activities, the levels of ROS, and mitochondrial membrane pote

RIP1K and RIP3K provoked by shikonin induce cell cycle arrest in the triple negative breast cancer cell line, MDA-MB-468: necroptosis as a desperate programmed...

Zahra Shahsavari, Fatemeh Karami-Tehrani, Siamak Salami, Mehran Ghasemzadeh
Journal PapersTumor Biology , Volume 37 , Issue 4, 2016 January , {Pages 4479-4491 }

Abstract

Resistance to cell death and reprogramming of metabolism are important in neoplastic cells. Increased resistance to apoptosis and recurrence of tumors are the major roadblocks to effective treatment of triple negative breast cancer. It has been thought that execution of necroptosis involves ROS generation and mitochondrial dysfunction in malignant cells. In this study, the effect of shikonin, an active substance from the dried root of Lithospermum erythrorhizon, on the induction of necroptosis or apoptosis, via RIP1K-RIP3K expressions has been examined in the triple negative breast cancer cell line. The expression levels of RIP1K and RIP3K, caspase-3 and caspase-8 activities, the levels of ROS, and mitochondrial membrane potential have been

Shikonin induced necroptosis via Reactive Oxygen Species in the T-47D breast cancer cell line

Zahra Shahsavari, Fatemeh Karami-Tehrani, Siamak Salami
Journal PapersAsian Pacific Journal of Cancer Prevention , Volume 16 , Issue 16, 2015 January , {Pages 7261-7266 }

Abstract

Breast cancer, the most common cancer in the women, is the leading cause of death. Necrotic signaling pathways will enable targeted therapeutic agents to eliminate apoptosis-resistant cancer cells. In the present study, the effect of shikonin on the induction of cell necroptosis or apoptosis was evaluated using the T-47D breast cancer cell line. The cell death modes, caspase-3 and 8 activities and the levels of reactive oxygen species (ROS) were assessed. Cell death mainly occurred through necroptosis. In the presence of Nec-1, caspase-3 mediated apoptosis was apparent in the shikonin treated cells. Shikonin stimulates ROS generation in the mitochondria of T-47D cells, which causes necroptosis or apoptosis. Induction of necroptosis, as a ba

Targeting prostate cancer cell metabolism: impact of hexokinase and CPT-1 enzymes

Rouhallah Najjar Sadeghi, Fatemeh Karami-Tehrani, Siamak Salami
Journal PapersTumor Biology , Volume 36 , Issue 4, 2015 April 1, {Pages 2893-2905 }

Abstract

Glycolysis has been shown to be required for the cell growth and proliferation in several cancer cells. However, prostate cancer cells were accused of using more fatty acid than glucose to meet their bioenergetic demands. The present study was designed to evaluate the involvement of hexokinase and CPT-1 in the cell growth and proliferation of human prostate cancer cell lines, PC3, and LNCaP-FGC-10. Hexokinase and CPT-1 activities were examined in the presence of different concentrations of their inhibitors, lonidamine and etomoxir, to find the concentration of maximum inhibition ([I max]). To assess cell viability and proliferation, dimethylthiazol (MTT) assay was carried out using [I

Apoptosis Induced by 13-S-hydroxyoctadecadienoic acid in the Breast Cancer Cell Lines, MCF-7 and MDA-MB-231

Masoumeh Tavakoli Yaraki, Fatemeh Karami-Tehrani
Journal PapersIran J Basic Med Sci , Volume 16 , Issue 4, 2013 April , {Pages 653- 659 }

Abstract

Objective (s): The 15-Lipoxygenase-1 (15-LOX-1) pathway has become of considerable interest as a promising molecular approach for the modulation of cancer cell growth. 13-S-hydroxyoctadecadienoic acid (13 (S)-HODE) is a main metabolite of 15-LOX-1 which is proposed to influence the cancer cell’s growth. This study aims to investigate the role of 13 (S)-HODE in the regulation of cell growth and apoptosis in the breast cancer cell lines.Materials and Methods: MTT assay was used to examine the cytotoxic effect of 13 (S)-HODE in the breast cancer cells, MCF-7 and MDA-MB-231. Annexin-V-FITC staining and cell cycle analysis were performed using flow cytometry. The effect of 13 (S)-HODE on the expression level of Peroxisome proliferator-activate

Induction of apoptosis by Trichostatin A in human breast cancer cell lines: involvement of 15-Lox-1

Masoumeh Tavakoli-Yaraki, Fatemeh Karami-Tehrani, Vahid Salimi, Majid Sirati-Sabet
Journal PapersTumor Biology , Volume 34 , Issue 1, 2013 February 1, {Pages 241-249 }

Abstract

15-Lipoxygenase-1 (15-Lox-1) is a key enzyme mediating oxidative metabolism of polyunsaturated fatty acids and has attracted considerable interest as a potential target for the induction of apoptosis in cancer cells. Knowledge of relationship between 15-Lox-1 and histone deacetylase inhibitors is lacking in the breast cancer. This study is aimed to investigate the role of Trichostatin A (TSA) and 13(S)-HODE, as a metabolite of 15-Lox-1, in the regulation of breast cancer cell growth. The cytotoxic effect of TSA, as a potent HDAC inhibitor, was measured using MTT assay. Annexin V–FITC and PI staining were performed to detect apoptosis and cell cycle distribution using Flow cytometry. The role of 15-Lox-1 in the regulation of

Inhibition of phosphodiestrase 9 induces c GMP accumulation and apoptosis in human breast cancer cell lines, MCF‐7 and MDA‐MB‐468

R Saravani, F Karami‐Tehrani, M Hashemi, M Aghaei, R Edalat
Journal PapersCell proliferation , Volume 45 , Issue 3, 2012 June , {Pages 199-206 }

Abstract

Objectives Phosphodiesterase 9 (PDE9) is a major isoform of phosphodiesterase hydrolysing cGMP and plays a key role in proliferation of cells, their differentiation and apoptosis, via intracellular cGMP signalling. The study described here was designed to investigate expression, activity and apoptotic effect of PDE9 on human breast cancer cell lines, MCF‐7 and MDA‐MB‐468. Materials and methods Activity and expression of PDE9 were examined using colorimetric cyclic nucleotide phosphodiesterase assay and real‐time RT‐PCR methods respectively; cGMP concentration was also measured. MTT viability test, annexin V‐FITC staining, Hoechst 33258 staining and caspase3 activity assay were used to detect apoptosis. Results Treatment of

Expression of cGMP-dependent protein kinase, PKGIα, PKGIβ, and PKGII in malignant and benign breast tumors

Fatemeh Karami-Tehrani, Faranak Fallahian, Morteza Atri
Journal PapersTumor Biology , Volume 33 , Issue 6, 2012 December 1, {Pages 1927-1932 }

Abstract

Cyclic GMP-dependent protein kinases (PKG) constitute a small family of enzymes that are encoded by two genes. Two major forms of PKG have been identified in mammalian cells, PKG I and PKG II. In addition, there are two splice variants of PKG I, which are designated as Iα and Iβ. There are increasing evidences that PKG can play an important role in the inhibition of cell proliferation and induction of apoptosis. In our previous studies, the inhibitory effects of cGMP/PKG on the cell growth were indicated using breast cancer cell lines. Accordingly, the present study was designed to compare the expression levels of three PKG isoforms in normal, benign, and malignant breast tissues. The expression level of PKG isoforms was as

Adenosine induces cell‐cycle arrest and apoptosis in androgen‐dependent and‐independent prostate cancer cell lines, LNcap‐FGC‐10, DU‐145, and PC3

Mahmoud Aghaei, Fatemeh Karami‐Tehrani, Mojtaba Panjehpour, Siamak Salami, Faranak Fallahian
Journal PapersThe Prostate , Volume 72 , Issue 4, 2012 March , {Pages 361-375 }

Abstract

BACKGROUND Adenosine has been shown to inhibit cell growth and induce apoptosis in the several cancer cells via intrinsic and extrinsic pathway. The present study was designed to understand the mechanism underlying adenosine‐induced apoptosis in the DU‐145, PC3, and LNcap‐FGC10 human prostate cancer cells. METHODS To observe cell viability and proliferation, MTT assay, cell counting, and BrdU assay were carried out in DU‐145, PC3, and LNcap‐FGC10 cells. Apoptosis was assessed with the analysis of cell cycle, Hoechst 33258 staining, propidium iodide and annexin‐V staining, reactive oxygen species (ROS) formation, mitochondrial membrane potential (ΔΨM) measurement, caspase‐3 activity assay, Bcl‐2 and Bax protein expressi

Evaluation of PDE5 and PDE9 expression in benign and malignant breast tumors

Fatemeh Karami-Tehrani, Marzieh Moeinifard, Mahmoud Aghaei, Morteza Atri
Journal PapersArchives of medical research , Volume 43 , Issue 6, 2012 August 1, {Pages 470-475 }

Abstract

Background and AimsPhosphodiesterases 5 and 9 (PDE5, PDE9) are enzymes responsible for regulating second messenger signaling by hydrolyzing 3’,5’ cyclic guanosine monophosphate (cGMP). PDE isoforms are deregulated in some types of human cancer. The present study was carried out to evaluate the expression of phosphodiesterase isoenzymes, PDE5 and PDE9, in benign and malignant breast tumors.MethodsThe expression levels of PDE5 and PDE9 were assayed in malignant and benign breast tumors and corresponding normal breast tissues using quantitative real-time RT-PCR. Moreover, the correlation between PDE5, PDE9 relative expression and clinicopathological characteristics were analyzed.ResultsThe relative expressions of PDE5 and PDE9 in malignant

/pro/academic_staff/karamitf/publication

دروس نیمسال جاری

  • دكتري
    بيماري هاي متابوليك مادرزادي ( واحد)
    دانشکده علوم پزشکی، گروه بيو شيمي پزشكي
  • دكتري
    بيوشيمي سرطان و تومور ماركرها ( واحد)

دروس نیمسال قبل

  • كارشناسي ارشد
    متابوليسم مواد سه گانه و اختلالات ( واحد)
    دانشکده علوم پزشکی، گروه بيو شيمي پزشكي
  • دكتري
    بيوشيمي سرطان و تومور ماركرها ( واحد)
  • 1399
    نيك اختر, صادق
    بررسي ميزان ABCC5 (MRP5) در سطح ژن و پروتئين، در نمونه هاي بافت بدخيم، خوش خيم و نرمال رحم
    داده ای یافت نشد
    داده ای یافت نشد

مهم

جدید

    اطلاعیه ای درج نشده است