Objective: Multiple myeloma (MM) is an incurable plasma cell malignancy. Several genetic and epigenetic changes affect numerous critical genes expression status in this disorder. CDKN2A gene is expressed at low level in almost all cases with MM disease. The mechanism of this gene down-regulation has remained controversial. In the present study, we targeted EZH2 by microRNA-124 (miR-124) in L-363 cells and assessed following possible impact on CDKN2A gene expression and phenotypic changes.Materials and Methods: In this experimental study, growth inhibitory effects of miR-124 were measured by MTT assay in L-363 cell line. Likewise, cell cycle assay was measured by flowcytometery. The expression levels of EZH2 and CDKN2A were evaluated by real

Background: Acute T lymphoblastic Leukemia (T-ALL) is a highly aggressive hematologic malignancy. Chemotherapy resistance is one of the most important challenges in T-ALL treatment. Alterations in cellular signaling pathways such as Notch1 and PI3K/AKT/mTOR play a role in cell proliferation, survival, and resistance to chemotherapy. Combination of Notch1 and PI3K/AKT/mTOR inhibitors is an interesting and rational strategy in treatment of T-ALL. Interaction of AZD5363 as an inhibitor of PI3k/AKT/mTOR and Compound E as an inhibitor of Notch1 signaling pathway was investigated in a T-ALL pre-clinical model.Materials and Methods: T-ALL cell lines included Jurkat, Molt-4, and HPB-ALL cells were treated with AZD5363 and Compound E alone and in co

The therapeutic properties of tin nanoparticles have been proved in recent years. One of their probable effects is anti‐anemia. In this study, tin nanoparticles were synthesized and characterized in aqueous medium using Ziziphora clinopodioides Lam leaf aqueous extract as the reducing and stabilizing agent. We also assessed the antihemolytic anemia potential of tin nanoparticles (SnNPs) in an animal model of hemolytic anemia. Tin nanoparticles were characterized using many techniques including Fourier transform‐infrared and UV‐visible spectroscopy, X‐ray diffraction (XRD), energy dispersive X‐ray spectrometry, and field emission‐scanning electron microscopy (FE‐SEM). FE‐SEM images showed a uniform spherical morphology in siz

Co-transplantation of MSCs and HSCs has showed controversial results for treatment of GvHD. In this study, the level of predictor factors and GvHD biomarkers have been checked. The co-transplantation of HSCs and MSCs did not show a significant difference compared to the group received MSCs alone.

: Differentiation of human mesenchymal stem cells (hMSC) to neural cells on Nano-scaffolds is a promising method for the treatment of the damaged nervous system through bionanomaterial-cell transplantation. The hMSC’s multipotential features have been discovered in various tissue engineering researches. This investigation shows the in-vitro development and neural differentiation of hMSC in 3D and 2D environments. The 3D environment which used in this study is nanofibrous polycaprolactone (PCL). The differentiation potential of mesenchymal stem cells (MSCs) to neural cells, on the random polycaprolactone (PCL) nanofibrous scaffolds, and tissue plate was examined. Researches have proved that interaction of extracellular nanofibrous matrix w

IntroductionThrombocytopenia is a common consequence of leukemia that affects the outcome of hematopoietic stem cell transplantation (HSCT). The stromal damage of bone marrow following pre-HSCT conditioning regimens can delay the hematopoietic engraftment and increased blood product transfusions. We tried to define threshold based on pre-transplant platelet count as a biomarker to predict engraftment time and blood product requirements in allogeneic HSCT patients.MethodsThis retrospective study was performed on 194 patients who received allogeneic HSCT. The median for platelet (PLT) count of patients at the admission day was considered as a cut off value. The association of platelet count with white blood cell (WBC) and PLT engraftment time

In the current work, the adsorption of Sulfanilamide (SLF) drug over B12N12 and Al12N12 fullerenes was studied using DFT and TDDFT calculations at the M06-2X/6-31+ G** level in the solvent water for the first time. The adsorption effect of the SLF on the bond length, electronic properties such as charge analysis, frontier molecular orbital (FMO), dipole moment and optical properties of B12N12 and Al12N12 fullerenes, was investigated. The UV absorption spectra were calculated for the study of the significant changes taking place in interactions between SLF and B12N12 and Al12N12 fullerenes. According to charge analysis, it is found that charge transfer occurs from SLF drug to fullerenes and from fullerene to SLF drug. The analysis of the LOL

Objective: In the present study, the interaction between drug Tyrphostin AG528 and CNT(6,6-6) nanotube by Density Functional Theory (DFT) calculations in solvent water has been investigated for the first time. Methods and Results: According to the calculations, intermolecular hydrogen bonds take place between an active position of the molecule Tyrphostin AG528 and hydrogen atoms of the nanotube which play an important role in the stability of complex CNT(6,6- 6)/Tyrphostin AG528. The non-bonded interaction effects of the molecule Tyrphostin AG528 with CNT(6,6-6) nanotube on the electronic properties, chemical shift tensors and natural charge have also been detected. The natural bond orbital (NBO) analysis suggested that the molecule Tyrphos

In this work, density functional theory (DFT) calculations were performed to investigate the complex formation ability of Meso-2,3-dimercaptosuccinic acid (DMSA) with metal ions (Cd2+, Hg2+ and Pb2+) in water. The binding energy values and thermodynamic parameters have been calculated. Natural bond orbital and charge decomposition analyses show an effective charge transfer from the oxygen and sulfur atoms of the DMSA to metal ions. Quantum theory of atoms in molecules analysis reveals that the covalent interactions between DMSA and Pb2+ are the driving force in complex formation, while the non-covalent interactions, mainly, electrostatic interactions play an important role in the complex formation of the DMSA with Cd2+ and Hg2+. Finally, Th

β‐Thalassemia is a common monogenic disease characterized by defective β‐globin chains synthesis. In vitro β‐thalassemia‐related research on increasing β‐like globin genes or identification of factors reducing the severity of the disease, has been performed on mouse erythroleukaemia or K562 cell lines. The aim of this study was the production of an in vitro model of β‐thalassemia using the highly efficient CRISPR‐Cas9 system. Embryonic stem (ES) cells were nucleofected with guide RNA (gRNA)‐Cas9 expression vectors. Molecular testing was done on extracted DNA to assess Hbb‐b1 mutation. Analysis of transcription factors and hemoglobin genes were evaluated using quantitative reverse transcription‐polymerase chain reac

The main purpose of this study is a better comprehension of the non-bonded interaction between an anticancer drug Ciclopirox and carbon nanotube [CNT (6, 6-6)]. The electronic structure and adsorption properties of the molecule Ciclopirox over the surface of CNT were theoretically studied in the solvent phase at the B3LYP/6-31G* level of theory for the first time. The electronic spectra of the Ciclopirox drug, CNT (6, 6-6) and complex CNT (6, 6-6)/Ciclopirox in solvent water were calculated by time dependent density functional theory (TD-DFT) for the investigation of adsorption effect. The non-bonded interaction effects of the Ciclopirox drug with CNT (6, 6-6) on the chemical shift tensors and natural charge have been also detected. Accordi

Background: Factor XIII Deficiency (FXIIID) is an inherited rare bleeding disorder with some life threatening clinical manifestation including Intracranial Haemorrhage (ICH). Among all polymorphisms found in FXIIID, Thrombin Activatable Fibrinolysis Inhibitor (TAFI) Thr325Ile gene polymorphism increases probability of ICH about 20 fold in patients with FXIII. So, in this study we aimed to evaluate TAFI Thr 325 Ile polymorphism in Chorionic villus samples (CVS) of fetuses with positive family history of FXIIID and ICH. Materials and Methods: This study was performed on chorionic villus of pregnant mothers with positive history of FXIIID accompanied with ICH in first-degree relatives of their fetus. All parents of the fetuses were completed c

Parvovirus B19 (B19V) has been known to induce transient erythroid aplasia, cytopenia and aplastic anemia. This virus persists in bone marrow mesenchymal stem cells (HBMSCs) of some immunocompetent individuals several years after primary infection. In B19V infected erythroid progenitor cells, the virus induces transactivation of Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) gene expression. Due the critical role of HBMSCs in bone marrow niche and inhibitory effect of inflammatory cytokines on hematopoiesis, the aim of this study was to investigate the effect of B19V on IL-6 and TNF-α gene expression intransfected cells. In addition we assessed the clonogenicity potential of cord blood CD34+ stem cells that were co

In the given research, the molecular structures of two new compounds, 4-((E)-3-(dimethylamino) styryl)-6-((E)-4-(dimethylamino) styryl) pyrimidine-2-amine (PM-1) and N-(4-((E)-3-(dimethylamino) styryl)-6-((E)-4-(dimethylamino) styryl) pyrimidine-2-yl)-4, 6-dichloro 1, 3, 5-1, 3, 5-triazin-2-amine (PM-2), have been studied with the use of density functional theory (DFT/B3LYP/MidiX) in dimethylformamide (DMF) for the first time. The electronic spectra of the new compounds in a DMF solvent were carried out by temporally dependent density functional theory (TD-DFT) method. The computed absorption spectral data of the title compounds are in good agreement with the experimental data, thus allowing an assignment of the UV/Vis spectra. The equilibr

The main purpose of this study is a better comprehension of the non-bonded interaction between an anticancer drug apalutamide and deoxyribonucleic acid (DNA). In the present work, the interaction between anticancer drug apalutamide and one of the DNA bases called 2'-deoxythymidine 5'-monophosphate (thymine) by Density Functional Theory (DFT) calculations in the solvent water has been investigated for the first time. The non-bonded interaction effects of the molecule apalutamide with thymine on the electronic properties, chemical shift tensors and natural charges have been also detected. The natural bond orbital (NBO) analysis was performed for determining the role of electron donor and acceptor of the molecules apalutamide and thymine at th

BackgroundRecent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with cancer progression and prognosis. Thus, we here determined MALAT1 gene expression in chronic lymphocytic leukemia (CLL), a genetically heterogeneous disease, and explored its possible relationships with cytogenetic abnormalities.MethodsMALAT1 expression level was evaluated using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on blood mononuclear cells from 30 non-treated CLL patients and 30 matched healthy controls. Cytogenetic abnormalities were determined in patients by fluorescence in situ hybridization (