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  • دکتری (1363)

    آسیب شناسی

    نیویورک، آمریکا

  • ترمیم ضایعات نخاعی
  • تمایز سلول های بنیادی به سلول‌های نورواپی تلیال

    نام ونام خانوادگی: تقی طریحی محل تولد: نجف عراق سال تولد : 1332 تحصیلات: لیسانس دامپزشکی و جراحی دامپزشکی دکتری تخصصی پاتولوزی (Ph.D) از دانشگاه بوفالو

    ارتباط

    رزومه

    PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells

    Marzieh Darvishi, Hatef Ghasemi Hamidabadi, Sajad Sahab Negah, Ardeshir Moayeri, Taki Tiraihi, Javad Mirnajafi-Zadeh, Ali Jahanbazi Jahan-Abad, Amir Shojaei
    Journal PapersIranian Journal of Basic Medical Sciences , Volume 23 , Issue 4, 2020 April 1, {Pages 431-438 }

    Abstract

    Objective(s): Cell therapy has provided clinical applications to the treatment of motor neuron diseases. The current obstacle in stem cell therapy is to direct differentiation of stem cells into neurons in the neurodegenerative disorders. Biomaterial scaffolds can improve cell differentiation and are widely used in translational medicine and tissue engineering. The aim of this study was to compare the efficiency of two-dimensional with a three-dimensional culture system in their ability to generate functional motor neuron-like cells from adipose-derived stem cells. Materials and Methods: We compared motor neuron-like cells derived from rat adipose tissue in differentiation, adhesion, proliferation, and functional properties on two-dimension

    Differential gene expression by lithium chloride induction of adipose-derived stem cells into neural phenotype cells

    Samaneh Farrokhfar, Taki Tiraihi, Mansoureh Movahedin, Hossein Azizi
    Journal PapersIranian Journal of Basic Medical Sciences , Volume 23 , Issue 4, 2020 April 1, {Pages 544-550 }

    Abstract

    Objective(s): Adipose-derived stem cells (ADSCs), with suitable and easy access, are multipotential cells that have the ability for differentiation into other mesodermal and transdifferentiate into neural phenotype cells. In this study, Lithium chloride (LiCl) was used for in vitro transdifferentiation of rat ADSCs into neuron-like cells (NLCs).Materials and Methods: ADSCs were isolated from the rats’ perinephric region using Dulbecco΄s Modified Eagle΄s Medium (DMEM) with Fetal Bovine Serum (FBS), cultured for 3 passages, characterized by flowcytometry and differentiation into adipogenic and osteogenic phenotypes. The ADSCs were exposed to 0.1, 0.5, 1, 1.5, 2, 5, and 10 millimolar (mM) LiCl without serum for 24 hr. The optimum dose of L

    Conversion of Neural Stem Cells into Functional Neuron-Like Cells by MicroRNA-218: Differential Expression of Functionality Genes

    Wissam Khalil, Taki Tiraihi, Masoud Soleimani, Nafiseh Baheiraei, Kazem Zibara
    Journal PapersNeurotoxicity Research , Volume 38 , Issue 3, 2020 October , {Pages 707-722 }

    Abstract

    Conversion of mesenchymal stem cells (MSC) into neuron-like cells (NLC) is a feasible cell therapy strategy for replacing lost neurons in neuronal disorders. In this study, adipose-derived MSC (ADMSC) were converted into neural stem cells (NSC) via neurosphere. The resulting NSC were then differentiated into NLC by transduction with microRNA-218, using a lentiviral vector. ADMSC, NSC, and NLC were first characterized by flow cytometry, RT-PCR, and immunocytochemistry. The functionality of the NLC was evaluated by qRT-PCR and patch clamp recording. Immunophenotyping of ADMSC showed their immunoreactivity to MSC markers CD90, CD73, CD105, and CD49d, but not to CD31 and CD45. RT-PCR results demonstrated the expression of nestin, neurogenin, ne

    Generation of Retinal Pigmented Epithelium-Like Cells from Pigmented Spheres Differentiated from Bone Marrow Stromal Cell-Derived Neurospheres

    Hamid Aboutaleb Kadkhodaeian, Taki Tiraihi, Hamid Ahmadieh, Hossein Ziaei, Narsis Daftarian, Taher Taheri
    Journal PapersTissue engineering and regenerative medicine , Volume 16 , Issue 3, 2019 June 1, {Pages 253-263 }

    Abstract

    Background: Retinal degeneration causes blindness, and cell replacement is a potential therapy. The purpose of this study is to formation of pigmented neurospheres in a simple medium, low-cost, high-performance manner over a short period of time while expressing markers of RPE cells and the activation of specific genes of the pigment cells. Also, these neurospheres have the ability to produce a monolayer of retinal pigment epithelium-like cells (RPELC) with the ability of photoreceptor outer segment phagocytosis. Methods: BMSC were isolated from pigmented hooded male rats and were immunoreactive to BMSC markers, then converted into neurospheres,

    Diagnosing Hand and Wrist Tendon Injuries in Patients with Questionable Physical Findings: Let POCUS Show Its True Mettle

    Reza Akhavan, Ehsan Bolvardi, Masoud Pezeshki-Rad, Bita Abbasi
    Journal PapersTrauma Monthly , Volume 24 , Issue 4, 2019 May 1, {Pages e67967-e67967 }

    Abstract

    Central Panel.

    Implications of immunotherapy with high-dose glatiramer acetate in acute phase of spinal cord injury in rats

    Hadi Askarifirouzjaei, Leila Khajoueinejad, Amir Salek Farrokhi, Mohammad-Taher Tahoori, Mehdi Fazeli, Taki Tiraihi, Ali Akbar Pourfathollah
    Journal PapersImmunopharmacology and immunotoxicology , Volume 41 , Issue 1, 2019 January 2, {Pages 150-162 }

    Abstract

    Objective: Recently, many researches with different viewpoints have focused on application of immunotherapy agents in treatment of spinal cord injury (SCI) according to neuroprotective results in some neurodegenerative disease. Glatiramer acetate (GA) is the most commonly used drug for Multiple sclerosis (MS) patients that exerts an immunomodulatory effect against Myelin basic protein (MBP) antigen.Materials and methods: High-dose (2mg/kg) treatment of GA for 28 consecutive days after SCI was compared with its low-dose (0.5 mg/kg) treatment, SCI control and Sham control rat groups.Results: High-dose GA group had significantly worsened outcome in standard functional recovery evaluation test (BBB) 12 weeks after SCI compared to SCI control

    Comparison of the proteome patterns of adipose-derived stem cells with those treated with selegiline using a two dimensional gel electrophoresis analysis

    M Mardani, T Tiraihi, SZ Bathaie, J Mirnajafi-Zadeh
    Journal PapersBiotechnic & Histochemistry , 2019 October 5, {Pages 10-Jan }

    Abstract

    Adipose derived stem cells (ADSCs) are multipotent and can transdifferentiate into neural stem cells. We investigated the transdifferentiation of ADSCs to neural phenotype (NP) cells using selegiline and two-dimensional electrophoresis (2-DE). The perinephric and inguinal fat of rats was collected and used to isolate ADSCs that were characterized by immunophenotyping using flow cytometry. The ADSCs were differentiated into osteogenic and lipogenic cells. The NP cells were generated using 10−9 mM selegiline and characterized by immunocytochemical staining of nestin and neurofilament 68 (NF-68), and by qRT-PCR of nestin, neurod1 and NF68. Total protein of ADSCs and NP cells was extracted and their proteome patterns were examined using 2-DE.

    Protein Kinase Cε in the Platelet and Hippocampal Tissue as a Diagnostic Biological Marker in Alzheimer Disease

    Sara Amiri, Kayhan Azadmaneshb, Marzieh Dehghan Shasaltaneh, Baharak Khoshkholgh-Sima, Nasser Naghdi
    Journal PapersBasic and Clinical Neuroscience , Volume 10 , Issue 6, 2019 November 10, {Pages 545-556 }

    Abstract

    Alzheimer disease (AD) is one of the most common neurodegenerative disorders. It is characterized by the loss of mental, behavioral, and functional abilities. Formation of amyloid plaques, tau hyperphosphorylation, and inflammation lead to synaptic impairment and destroying the integrity of brain functions (Kumar, Singh, & Ekavali, 2015). Pyramidal cells of the entorhinal cortex and the CA1 region of the hippocampus are destructed in Alzheimer brains (Huang & Mucke, 2012), which may be resulted from the deficiency of several enzymes in the mentioned regions, such as protein kinase C (PKC) and mitogen-activated protein kinase (MAPK)(Hong-Qi, Zhi-Kun, & Sheng-Di, 2012). PKC alters in fibroblasts, lymphocytes and red blood cells of AD patients

    Trans-Differentiation of Human Dental Pulp Stem Cells Into Cholinergic- Like Neurons Via Nerve Growth Factor

    Shahram Darabi, Taki Tiraihi, Maryam Nazm Bojnordi, Hatef Ghasemi Hamidabadi, Nourollah Rezaei, Maria Zahiri, Rafieh Alizadeh
    Journal PapersBasic and Clinical Neuroscience , Volume 10 , Issue 6, 2019 November 10, {Pages 609-618 }

    Abstract

    Nowadays, cell therapy is highly regarded as one of the therapeutic methods for nervous system injuries (Naghdi et al., 2009; Darabi et al., 2013). Different types of cells, such as embryonic stem cells (ESCs), adult stem cells, and umbilical cord stem cells have been studied for transplantation into the nervous system (Boncoraglio et al., 2010; Bojnordi et al., 2012). The cholinergic neurons are used for the treatment of motor neuron degeneration (Abdanipour et al., 2014; Bojnordi et al., 2013) and Alzheimer disease (Thonhoff et al., 2009).In previous studies, cholinergic neurons have been generated from ESCs and induced pluripotent stem cells (iPSCs). Because of the allogeneic, tumorigenic, and ethical problems, using other mesenchymal st

    Trans-Differentiation of Human Dental Pulp Stem Cells Into Cholinergic-Like Neurons Via Nerve Growth Factor

    Shahram Darabi, Taki Tiraihi, Maryam Nazm Bojnordi, Hatef Ghasemi Hamidabadi, Nourollah Rezaei, Maria Zahiri, Rafieh Alizadeh
    Journal PapersBasic and Clinical Neuroscience , Volume 10 , Issue 6, 2019 November , {Pages 609 }

    Abstract

    Methods:The hDPSCs were evaluated for CD73, CD31, CD34, and Oct-4. Concentration-time relationships for NGF were assessed by evaluating the viability rate of cells and the immune response to nestin, neurofilament 160, microtubule-associated protein-2, and choline acetyltransferase.Results:The hDPSCs had a negative response to CD34 and CD31. The optimal dose for the NGF was 50 ng/mL seven days after the induction when the highest percentage of expressing markers for the Cholinergic neurons (ChAT) was detected.Conclusion:The results of this study provided a method for producing cholinergic neurons by hDPSCs, which can be used in cytotherapy for degenerative diseases of the nervous system and also spinal cord injury.

    Characterization of lumbar lymph node, a CNS-specific draining lymph node, after spinal cord injury in rats

    Hadi Askarifirouzjaei, Leila Khajoueinejad, Mehdi Fazeli, Taki Tiraihi, Ali Akbar Pourfathollah
    Journal Papers , 2019 November 15, {Pages }

    Abstract

    Regional tissue-draining lymph nodes are the major active site after inflammation that generate primary immune responses according to lymphatic system pathways. However, CNS specific draining lymph nodes have not characterized individually after spinal cord injury (SCI). Therefore, we examined the morphofunctional state of the Lumbar lymph node, the closest CNS-draining lymph node adjacent to the lesion site in thoracic spinal cord injury. Findings: Lumbar lymph nodes isolated 7 days after low thoracic spinal cord injury were compared with sham control and intact control rats. The significant enlargement was observed in lumbar lymph nodes in SCI group accompanied by increased total cell number, while there was a significantly higher cell de

    Nerve Growth Factor and the Trans-differentiation of Human Dental Pulp Stem Cells Into Cholinergic-like Neurons

    Shahram Darabi, Taki Tiraihi, Maryam Nazm Bojnordi, Hatef Ghasemi Hamidabadi, Nourollah Rezaei, Maria Zahiri, Rafieh Alizadeh
    Journal PapersBasic and Clinical Neuroscience , Volume 10 , Issue 6, 2019 November 10, {Pages 07-Jul }

    Abstract

    Introduction: Cell therapy has been widely considered as a therapeutic approach for neurodegenerative diseases and nervous system damage. Cholinergic neurons as one of the most important neurons that play a significant role in controlling emotions, mobility, and autonomic systems. In this study, human dental pulp stem cells (hDPSCs) were differentiated into the cholinergic neurons by β-mercaptoethanol in the preinduction phase and also by the nerve growth factor (NGF) in the induction phase.Methods: The hDPSCs were evaluated for CD73, CD31, CD34, and Oct-4. Concentration-time relationships for NGF were assessed by evaluating the viability rate of cells and the immune response to nestin, neurofilament 160, microtubule-associated protein-2,

    Protein Kinase Cɛ in the Platelet and Hippocampal Tissue as a Diagnostic Biological Marker in Alzheimer Disease

    Sara Amiri, Kayhan Azadmanesh, Marzieh Dehghan Shasaltaneh, Baharak Khoshkholgh-Sima, Nasser Naghdi
    Journal PapersBasic and Clinical Neuroscience , Volume 10 , Issue 6, 2019 November , {Pages 545 }

    Abstract

    Methods:In this study, spatial learning and memory for treated rats were evaluated by the Morris water maze test. The activity (total PKC), mRNA expression, and protein level of PKCɛ in the platelet and hippocampal tissue were evaluated using immunosorbent assay, real-time qPCR, and western blotting analysis, respectively.Results:The traveled distance was significantly prolonged, and escape latency significantly increased in Aβ-treated groups. PKC activity assay showed that there was a remarkable difference between the Aβ-treated and sham-operated groups on days 10 and 30 in the hippocampus and also day 30 in platelet after the injection of Aβ. A significant effect in PKC activity was observed between days 0 and 10, days 0 and 30, as we

    Characterization of lumbar lymph node, a CNS-specific draining lymph node, after spinal cord injury in rats

    AA Pourfathollah, H Askarifirouzjaei, L Khajoueinejad, M Fazeli, T Tiraihi
    Journal Papers , 2019 November 15, {Pages }

    Abstract

    Regional tissue-draining lymph nodes are the major active site after inflammation that generate primary immune responses according to lymphatic system pathways. However, CNS specific draining lymph nodes have not characterized individually after spinal cord injury (SCI). Therefore, we examined the morphofunctional state of the Lumbar lymph node, the closest CNS-draining lymph node adjacent to the lesion site in thoracic spinal cord injury. Findings: Lumbar lymph nodes isolated 7 days after low thoracic spinal cord injury were compared with sham control and intact control rats. The significant enlargement was observed in lumbar lymph nodes in SCI group accompanied by increased total cell number, while there was a significantly higher cell de

    Immunocytochemical analysis of valproic acid induced histone H3 and H4 acetylation during differentiation of rat adipose derived stem cells into neuron-like cells

    F Rezaei, T Tiraihi, A Abdanipour, HK Hassoun, T Taheri
    Journal PapersBiotechnic & Histochemistry , Volume 93 , Issue 8, 2018 November 17, {Pages 589-600 }

    Abstract

    Valproic acid (VPA) is an inhibitor of histone deacetylases (HDACs) that can regulate differentiation and proliferation of stem cells by epigenetic mechanisms. We investigated VPA induced histone H3 and H4 acetylation in adipose derived stem cells (ADSCs) transdifferentiated into neuron-like cells (NLCs). Rat ADSCs were transdifferentiated into neural stem cells (NSCs) that had been generated from neurospheres. The NSCs were differentiated into NLCs by induction with different concentrations of VPA at 24, 48 and 72?h. The NLCs were evaluated using anti-H3 and -H4 antibodies, and ADSCs, NSCs and NLCs were evaluated using immunofluorescence. The ADSCs were immunoreactive to CD90 and CD49d, but not to CD45 and CD31. Both the neurospheres and N

    Combined effects of 3D bone marrow stem cell-seeded wet-electrospun poly lactic acid scaffolds on full-thickness skin wound healing

    Sadegh Ghorbani, Hossein Eyni, Taki Tiraihi, Leila Salari Asl, Masoud Soleimani, Amir Atashi, Shahram Pour Beiranvand, Majid Ebrahimi Warkiani
    Journal PapersInternational Journal of Polymeric Materials and Polymeric Biomaterials , Volume 67 , Issue 15, 2018 October 13, {Pages 905-912 }

    Abstract

    Tissue engineering has emerged as an alternative treatment to traditional grafts for skin wound healing. Three-dimensional nanofibers have been used extensively for this purpose due to their excellent biomedical-related properties. In this study, high porous 3D poly lactic acid nanofibrous scaffolds (PLA-S) were prepared by wet-electrospinning technique and seeded with rat bone-marrow stem cells (BMSCs) to characterize the biocompatibility and therapeutic efficacy of these fibers on the treating full-thickness dermal wounds. The results of in vitro andin vivo studies indicate that the 3D fibrous PLA-S can be a potential wound dressing for wound repair, particularly when seeded with BMSCs.

    The Effects of Mozart’s Music on Interictal Activity in Epileptic Patients: Systematic Review and Meta-analysis of the Literature

    Samaneh Sadat Dastgheib, Parvaneh Layegh, Ramin Sadeghi, Ali Gorji, Fariba Karimzadeh, Mansoureh Soleimani, Mehdi Mehdizadeh, Payam Mohammad-Gharibani, Taki Tiraihi, Alireza Delshad, Taher Taheri, Atefeh Aminian, Farshid Noorbakhsh, Mahmoud Ghazi-Khansari
    Journal PapersIranian Journal of Basic Medical Sciences , Volume 21 , Issue 11, 2018 September , {Pages }

    Abstract

    ResearchGate is a network dedicated to science and research. Connect, collaborate and discover scientific publications, jobs and conferences. All for free.

    Decrease in cavity size and oligodendrocyte cell death using neurosphere-derived oligodendrocyte-like cells in spinal cord contusion model

    Hojjat Allah Abbaszadeh, Taki Tiraihi, Yousef Sadeghi, Ali Reza Delshad, Majid Sadeghizadeh, Taher Taheri, Ali Noori-Zadeh
    Journal PapersIranian biomedical journal , Volume 22 , Issue 4, 2018 July , {Pages 246 }

    Abstract

    Background:Oligodendrocyte cell death is among the important features of spinal cord injury, which appears within 15 min and occurs intensely for 4 h after injury, in the rat spinal contusion model. Accordingly, the number of oligodendrocytes progressively reduced within 24 h after injury. Administration of oligodendrocyte-like cells (OLCs) into the lesion area is one of the approaches to counterbalance this condition.Methods:Bone marrow stromal cells were transdifferentiated into neurospheres and then into neural stem cells and later were differentiated into OLCs using triiodothyronine and transplanted into the spinal cord contusion rats. The post-injury functional recovery was explored and compared with the control group using Basso-Beatt

    Differentiation of mesenchymal stem cells into neuron-like cells using composite 3D scaffold combined with valproic acid induction

    Sadegh Ghorbani, Taki Tiraihi, Masoud Soleimani
    Journal PapersJournal of biomaterials applications , Volume 32 , Issue 6, 2018 January , {Pages 702-715 }

    Abstract

    The nervous system has little capacity for self-repair after injury because neurons cannot proliferate owing to lack of suitable microenvironment. Therefore, neural tissue engineering that combines neural stem, scaffolds, and growth factors may improve the chance of restoration of damaged neural tissues. A favorable niche for neural regeneration would be both fibrous and electrically conductive scaffolds. Human Wharton jelly-derived mesenchymal stem cells were seeded on wet-electrospun 3D scaffolds composed of poly lactic acid coated with natural polymers including alginate and gelatin, followed by a multi-wall carbon nanotube coating. The results show that a wet-electrospun poly lactic acid scaffold at a concentration of 15% w/v had higher

    Poly-l-lysine-coated superparamagnetic nanoparticles: a novel method for the transfection of pro-BDNF into neural stem cells

    Salim Albukhaty, Hossein Naderi-Manesh, Taki Tiraihi, Majid Sakhi Jabir
    Journal PapersArtificial cells, nanomedicine, and biotechnology , Volume 46 , Issue sup3, 2018 November 12, {Pages S125-S132 }

    Abstract

    Poly-l-lysine-coated superparamagnetic iron oxide nanoparticles (SPIONs-PLL) were prepared and used as a novel-carrier for the transfer of brain-derived neurotrophic factor (BDNF) into neural stem cells (NSCs) under the beneficial influence of an external magnetic field. Pro-BDNF, a gene from human brain cDNA libraries, was obtained by polymerase chain reaction and constructed in a mammalian expression vector (PSecTag2/HygroB). The nanoparticles (NPs) were examined using Fourier transform infrared spectroscopy, zeta potential, and Transmission electron microscopy. From the results, the levels of BDNF among the transfected and untransfected cells were 30.326 ? 5.9 and 5.85 ? 3.11 pg/mL, respectively, as detected by an ELISA method.

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    دروس نیمسال جاری

    • دكتري
      روش هاي مطالعه بافتي ( واحد)
      دانشکده علوم پزشکی، گروه علوم تشريح
    • دكتري
      روش هاي مطالعه بافتي ( واحد)
    • دكتري
      تكنيكهاي پيشرفته ميكروآناتومي ( واحد)
    • دكتري
      تكنيكهاي پيشرفته ميكروآناتومي ( واحد)
    • كارشناسي ارشد
      تكنيك هاي پيشرفته علوم تشريحي ( واحد)
    • كارشناسي ارشد
      تكنيك هاي پيشرفته علوم تشريحي ( واحد)

    دروس نیمسال قبل

    • دكتري
      سلولهاي بنيادي ( واحد)
      دانشکده علوم پزشکی، گروه علوم تشريح
    • دكتري
      سلولهاي بنيادي ( واحد)
    • كارشناسي ارشد
      بيوشيمي ( واحد)
    • كارشناسي ارشد
      تكنيكهاي پيشرفته علوم تشريحي ( واحد)
    • كارشناسي ارشد
      تكنيكهاي پيشرفته علوم تشريحي ( واحد)
    • كارشناسي ارشد
      زيست شناسي سلولي و مولكولي ( واحد)
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      ملكي, معصومه
      كشت سلول هاي بنيادي مزانشيمي غده پاروتيد موش صحرايي انكپسوله شده در داربست هيدروژل آلژينات
    • 1398
      خادمي سيدبنادكي, سيد عماد
      درمان آسيب نخاعي كانتيوژني با استفاده از هيدروژل آلژينات/كيتوزان براي كپسوله‌ كردن سلول‌هاي شبه عصبي و انتقال ليپوزوم‌هاي حاوي miR-218 در موش صحرايي
      داده ای یافت نشد
      داده ای یافت نشد

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