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  • دکتری (1370)

    بیوشیمی بالینی

    علوم پزشکی تهران، تهران، ایران

  • کارشناسی‌ارشد (1365)

    بیوشیمی بالینی

    دانشگاه تربیت مدرس، تهران، ایران

  • بیوشیمی سلولی : بیماریها و اختلالات کبد (با تمرکز بر فیبروز و سیروز کبدی ) ، سلول درمانی و ژن درمانی
  • بیوشیمی مولکولی : نقص یا کمبودآلفا 1 –آنتی تریپسین ( AAATD) و بیماریهای وابسته به آن (اختلالات کبد ، ریه ، کلیه ....)
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رزومه

Breast Cancer Cell Lines, HER2/Neu Phenotype, and a Higher Propensity to Reactive Oxygen Species Production

Zahra Mohammadi Abgarmi, Abbas Sahebghadam Lotfi, Saeid Abroun, Masoud Soleimani, Shahla Mohammad Ganji, Parvaneh Baktash, Arash Moradi
Journal PapersArchives of Breast Cancer , 2021 April 15, {Pages 137-142 }

Abstract

Background: The reactive oxygen species (ROS) generated in the biological systems play an important role in pathological conditions and specific normal cellular processes, such as signaling pathways and drug sensitivity.Methods: Intracellular ROS was determined using (2'-7'dichlorofluorescin diacetate) DCFH-DA fluorimetric probe, malondialdehyde as lipid peroxidation index detected by the Thiobarbituric acid reactive substances method. Cells' protein carbonyl contents were assessed with 2, 4-Dinitrophenylhydrazine (DNPH) derivatization spectrophotometrically at 360-385 nm.Results: The oxidative stress induces ROS (p? 0.05), MDA (Malondialdehyde)(p? 0.05), and protein carbonylation (p? 0.01) was significantly higher in HER2-positive BT-474,

Raman Spectroscopic Characterization of Hepatic Differentiation of Mesenchymal Stem Cells

S Arjmand, A Barzegar, A Mohammadpour, H Rezaei, N Davoodian, ...
Journal Papers , , {Pages }

Abstract

Astaxanthin reduces demyelination and oligodendrocytes death in a rat model of multiple sclerosis

A Lotfi, M Soleimani, N Ghasemi
Journal Papers , , {Pages }

Abstract

A robust feeding control strategy adjusted and optimized by a neural network for enhancing of alpha 1-antitrypsin production in Pichia pastoris

Tina Tavasoli, Sareh Arjmand, Seyed Omid Ranaei Siadat, Seyed Abbas Shojaosadati, Abbas Sahebghadam Lotfi
Journal PapersBiochemical engineering journal , Volume 144 , 2019 April 15, {Pages 18-27 }

Abstract

Exact monitoring of the key process variables, such as specific growth rate (μ), is essential for effective process control, and maintenance of the optimal conditions for the production of recombinant proteins. In this study, a novel and confident on-line μ-stat approach for setting up methanol feeding strategy is described that is based on the consumption of ammonium hydroxide as feedback control. For this purpose a new and robust control with a time-based stable control structure was used, which had the ability to reconstruct the controller. Moreover, the MLP3 neural network was applied to adjust and optimize the performance of the robust control system. To evaluate the cited strategy, the overproduction of human recombinant alpha 1-ant

Comparison of transduction efficiency among various cell types by a lentivector containing CMV promoter

Hori Ghaneialvar, Abbas Sahebghadam Lotfi, Sareh Arjmand, Leila Soltani, Azra Kenarkoohi, Mohammad Mahdi Zangeneh
Journal PapersComparative Clinical Pathology , 2019 January , {Pages 09-Jan }

Abstract

Due to their unique biological characteristics, mesenchymal stem cells (MSCs) are useful in developing cell-based therapies, tissue regeneration, and many other applications of cellular and molecular genetics. Use of lentiviral system allows the integration of specific nucleic acid sequence into a target cell genome near an active transcription site in such a way that it is stable and heritable. The aim of the present study was to evaluate the gene transduction efficiency on the cells of different species using lentivirus along with cytomegalovirus in a lentiviral shuttle vector. In this study, the third-passage isolated MSCs from four different species, including ovine and goat cardiac fibroblasts, HEK293T, and L929 cell line were transduc

Aptamer-based sandwich assay for measurement of thymidine kinase 1 in serum of cancerous patients

M Nazari, SLM Gargari, A Sahebghadam Lotfi, MJ Rassaee, RA Taheri
Journal Papers , , {Pages }

Abstract

Characterization and Classification of Mesenchymal Stem Cells in Several Species Using Surface Markers for Cell Therapy Purposes

Hori Ghaneialvar, Leila Soltani, Hamid Reza Rahmani, Abbas Sahebghadam Lotfi, Masoud Soleimani
Journal PapersIndian Journal of Clinical Biochemistry , Volume 33 , Issue 1, 2018 January 1, {Pages 46-52 }

Abstract

Mesenchymal stem cells are multipotent cells capable of replicating as undifferentiated cells, and have the potential of differentiating into mesenchymal tissue lineages such as osteocytes, adipocytes and chondrocytes. Such lineages can then be used in cell therapy. The aim of present study was to characterize bone marrow derived mesenchymal stem cells in four different species, including: sheep, goat, human and mouse. Human bone-marrow mesenchymal stem cells were purchased, those of sheep and goat were isolated from fetal bone marrow, and those of mouse were collected by washing bone cavity of femur and tibia with DMEM/F12. Using flow-cytometry, they were characterized by CD surface antigens. Furthermore, cells of third pass

Promoting hepatogenic differentiation of human mesenchymal stem cells using a novel laminin-containing gelatin cryogel scaffold

Alemeh Mohammadpour, Sareh Arjmand, Abbas Sahebghadam Lotfi, Hossein Tavana, Maryam Kabir-Salmani
Journal PapersBiochemical and biophysical research communications , Volume 507 , Issue 04-Jan, 2018 December 9, {Pages 15-21 }

Abstract

Liver transplantation is the only definitive treatment currently available for acute and chronic liver failure. However, this approach has been restricted by complications including rejection and infection. Tissue engineering approaches using stem cell-derived functional hepatic cells offer a potential alternative. Using biologically compatible scaffolds is an important complementary key to achieve optimal construct for hepatic replacement. In the present study, to optimize the differentiation of human adipose-derived mesenchymal stem cells (ADMSCs) toward hepatocyte-like cells, a previously described gelatin cryogel was optimized and improved by laminin, the major component of basal lamina. The ADMSCs seeded on the scaffold displayed incre

Intraperitoneal immunization with Urease loaded N-trimethyl Chitosan nanoparticles elicits high protection against Brucella melitensis and Brucella abortus infections

Morteza Abkar, Mahdi Fasihi-Ramandi, Hamid Kooshki, Abbas Sahebghadam Lotfi
Journal PapersImmunology letters , Volume 199 , 2018 July 1, {Pages 53-60 }

Abstract

Brucella (B) species are brucellosis causative agents, a worldwide zoonotic illness causing Malta fever in humans and abortion in domestic animals. In this work, we evaluated the vaccine potential of Trimethyl chitosan (TMC) nanoparticles formulation of Urease (TMC/Urease) against brucellosis. TMC/Urease nanoparticles and urease without any adjuvant were separately administered both orally and intraperitoneally. Intraperitoneal (i.p.) administration of urease alone as well as oral administration of both TMC/Urease nanoparticles and urease alone, elicited low titers of specific immunoglobulin G (IgG), while i.p. immunization with TMC/Urease nanoparticles induced high specific IgG production levels. As it was indicated by the cytokine assay a

The combinational effect of E6/E7 siRNA and anti-miR-182 on apoptosis induction in HPV16-positive cervical cells

Hamidreza Javadi, Abbas Sahebghadam Lotfi, Saman Hosseinkhani, Hossein Mehrani, Jafar Amani, Zahra Soheila Soheili, Zahra Hojati, Mehdi Kamali
Journal PapersArtificial cells, nanomedicine, and biotechnology , 2018 June 5, {Pages 10-Jan }

Abstract

In the present research, we assumed that reducing the amounts of E6 and E7 oncoproteins by a specific siRNA sequence and recovering p53 and RB proteins, along with the recovery of the FOXO1 protein by applying anti-miR-182, would increase apoptosis and reduce proliferation rate in cancer cells. The HPV16-positive CaSki cervical cancer cell line was used. 48 hours after transfection of siRNA for targeting E6 and E7 oncoproteins and anti-miR-182, expression of its cellular targets p53, p21 and FOXO1 was assessed by real-time PCR, western blot analysis and immunocytofluorescence staining. In all treatments, apoptosis rate and viability were evaluated using Annexin-V-FITC apoptosis detection kits and MTT assays, respectively. Among the design

Inhibition of Erbb2 by Trastuzumab Induces Oxidative Stress Markers in HER2 Positive Breast Cancer Cell Lines

Zahra Mohammadi Abgarmi, Abbas Sahebghadam Lotfi, Saeid Abroun, Masoud Soleimani, Shahla Moahammad Ganji
Journal PapersJournal of Research in Medical and Dental Science , Volume 6 , Issue 3, 2018 May 2, {Pages 158-165 }

Abstract

Background: Human epidermal growth factor receptor2 (HER2) amplification occurs in approximately 30% of breast cancers that associated with a faster rate of growth and a poorer prognosis. Trastuzumab is FDA-approved humanized monocholonal antibody that targets the extracellular domain of HER2. In present study we blocked HER2 signaling pathways with trastuzumab and assessed oxidative stress markers in breast cancer cell lines.Methods: After MTT test, IC50 of trastuzumab on breast cancer cells were calculated with CompuSyn software. Cells treated with various concentration of trastuzumab. Intracellular ROS determined with DCFH-DA fluorimetric probe, MDA as lipid peroxidation index detected with TBARS method. Protein carbonyl contents of cell

Cell attachment evaluation of the immobilized bioactive peptide on a nanographene oxide composite

Behzad Adibi-Motlagh, Abbas Sahebghadam Lotfi, Aram Rezaei, Ehsan Hashemi
Journal PapersMaterials Science and Engineering: C , Volume 82 , 2018 January 1, {Pages 323-329 }

Abstract

The immobilization of bioactive peptides as key molecules in numerous biological and physiological functions holds promise for designing advanced biomaterials. Graphene and its derivatives, having unique physicochemical properties, have brought considerable attention in the life sciences. In this regard, the chemical manipulation of the graphene surface with bioactive peptides opens a new horizon to design bioactive materials for a variety of future nanobiotechnologies. In this study, the first straightforward strategy for the covalent immobilization of the cell-adhesion peptide onto the graphene surface based on the Ugi four-component assembly process (Ugi 4-CAP) will be presented. The modified adhesion motif peptide, as an amine component

Oral immunization of mice with Omp31-loaded N-trimethyl chitosan nanoparticles induces high protection against Brucella melitensis infection

Morteza Abkar, Mahdi Fasihi-Ramandi, Hamid Kooshki, Abbas Sahebghadam Lotfi
Journal PapersInternational journal of nanomedicine , Volume 12 , 2017 January , {Pages 8769 }

Abstract

Brucellosis is a group of closely associated zoonotic bacterial illnesses caused by members of the genus Brucella. B. melitensis Omp31 is a promising candidate for a subunit vaccine against brucellosis. This study surveyed the immunogenicity of Omp31 alone and with incomplete Freund’s adjuvant (Omp31-IFA) and N-trimethyl chitosan (TMC/Omp31) nanoparticles (NPs), as well as the effect of Omp31 immunization route on immunological responses and protection. After expression and purification, the recombinant Omp31 (rOmp31) was loaded onto TMC NPs by ionic gelation. The particle size, loading efficiency and in vitro release of the NPs were examined. Omp31-IFA was administered intraperitoneally, while TMC/Omp31 NPs were administered orally and i

Investigation of Sodium Dithionite Residues in the Rock Candies Produced in the Candies Making Plants of Hamadan, Iran

Abdolmotaleb Seidmohammadi, Gorban Asgari, Abbas Lotfi, Javad Fardmal, Ali Heshmati, Amin Pirmoghani
Journal PapersJournal of health research in community , Volume 3 , Issue 1, 2017 June 15, {Pages 08-Jan }

Abstract

Introduction and purpose: Rock candy is one of the most widely used sugar products in Iran. The use of chemical additives in food products can be a serious threat to human health and environment if it is not accompanied with sufficient monitoring and management. Regarding this, the present study was conducted with the aim of investigating the amount of sodium dithionite residue in the rock candies produced in the candy making plants of Hamadan, Iran. Methods: This descriptive and analytical study was conducted on the rock candies produced in all the active candy making plants of Hamadan in 2015. The sampling was performed for four months in 20 rock candy producing plants. The concentration of sodium dithionite residue was determined accordi

Adipose derived mesenchymal stem cells improve diabetic wound healing in mouse animal model: extracellular matrix remodeling maybe a potential therapeutic usage of stem cells

Hori Ghaneialvar, Abbas Sahebghadam Lotfi, Sareh Arjm, Masoud Soleimani, Fatemeh Mashhadi Abbas
Journal PapersBiomedical Research , Volume 28 , Issue 8, 2017 May 3, {Pages 3672-3679 }

Abstract

Skin wounds cause damage to the body's first layer of protection. This, disclosed a person to further injury. Wounds normally heal in a very orderly and efficient process. However, activation of this efficient process is sometimes lost in pathologic conditions such as diabetes. The objective of the present study was to evaluate the expression of some genes in Adipose Derived Mesenchymal Stem Cells (ADSCs) that were used for the healing of the diabetic wound of mouse. ADSCs were separated from adipose tissue of mice, confirmed by surface markers CD34, CD105, CD44 and bone or fat cells differentiation. Then 10 ? 105 stem cells were immediately injected in four areas around the wound that previously were created on the dorsal skin of each diab

List Of Journal Paper (s)

KIANA KARIMIFAR, HIVA ALIPANAH, MOHAMMAD REZA BIGDELI, SAMANEH DEHGHAN, KALANTARI ROSHANAK REZAEI, ALI AZARI, HORI GHANEIALVAR, SAREH ARJMAND, LOTFI ABBAS SAHEBGHADAM, MASOUD SOLEIMANI, ABBASS FATEMEH MASHHADI, TILAKI RAMEZANALI DIANATI, MOTLAGH SEYEDEHSA
Journal PapersJOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES , Volume 27 , Issue 148, 2017 May , {Pages }

Abstract

Journal articles indexed in JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES 148 Free download full-text articles in the Journals.

Influence of Adipose Derived Mesenchymal Stem Cells on the Effective Inflammatory Factors of Diabetic Wound Healing in Animal Models

Hori Ghaneialvar, Sareh Arjmand, Abbas Sahebghadam Lotfi, Masoud Soleimani, Fatemeh Mashhadi Abbas
Journal PapersJournal of Mazandaran University of Medical Sciences , Volume 27 , Issue 148, 2017 May 15, {Pages 21-Dec }

Abstract

Background and purpose: Chronic non-healing diabetic wounds are a major medical challenge worldwide. Stem cell therapy has been developed as a new approach in the management of such wounds that need to be explored further. In the present study we aimed to evaluate the effects of adipose derived mesenchymal stem cells (ADSCs) on gene expression level of some important inflammatory factors involved in wound healing in diabetic animal model.Materials and methods: ADSCs were isolated from adipose tissue of mice and their stemness was identified in terms of specific cell surface markers (CD44, CD90, and CD34) and multi-lineage differentiation potentials. Then 10 6 stem cells were injected in dermis area around the wounds and the wound tissues we

Necrosis of the thumb after inadvertent injection of diclofenac in the radial artery: a case report

Hyun-Chul Shon, Ji-Kang Park, Sang-Woo Kang, Jae-Young Yang
Journal PapersJournal of pain research , Volume 10 , 2017 January , {Pages 2803 }

Abstract

Inadvertent intra-arterial drug injection occurs rarely, but it can cause very serious clinical complications, and thus, awareness of inadvertent intra-arterial drug injection is needed. The complications mainly result from iatrogenic reasons and can occur because of vascular variations in the arteries, under circumstances where normal intravenous injection is difficult, or in drug abusers who perform self-injection. The adverse effect associated with intra-arterial drug injection is serious and may lead to necrosis, requiring amputation of the affected extremity, infection, pseudoaneurysm, rhabdomyolysis, compartment syndrome, and permanent disability. However, the etiology of such adverse effects has not been clearly identified and treatm

The enhancement of differentiating adipose derived mesenchymal stem cells toward hepatocyte like cells using gelatin cryogel scaffold

Maryam Ghaderi Gandomani, Abbas Sahebghadam Lotfi, Dormohammad Kordi Tamandani, Sareh Arjmand, Shaban Alizadeh
Journal PapersBiochemical and biophysical research communications , Volume 491 , Issue 4, 2017 September 30, {Pages 1000-1006 }

Abstract

Liver tissue engineering creates a promising methodology for developing functional tissue to restore or improve the function of lost or damaged liver by using appropriate cells and biologically compatible scaffolds. The present paper aims to study the hepatogenic potential of human adipose derived mesenchymal stem cells (hADSCs) on a 3D gelatin scaffold in?vitro. For this purpose, mesenchymal stem cells were isolated from human adipose tissue and characterized by flowcytometry analysis and mesodermal lineage differentiation capacity. Then, porous cryogel scaffolds were fabricated by cryogelating the gelatin using glutaraldehyde as the crosslinking agent. The structure of the scaffolds as well as the adhesion and proliferation of the cells w

Enhancement of Alpha 1-antitrypsin Production in Pichia pastoris by Designing and Optimizing Medium Using Elemental Analysis

Tina Tavasoli, Sareh Arjmand, Seyed Omid Ranaei Siadat, Seyed Abbas Shojaosadati, Abbas Sahebghadam Lotfi
Journal PapersIranian journal of biotechnology , Volume 15 , Issue 4, 2017 January , {Pages 224 }

Abstract

Background: Human alpha 1-antitrypsin (AAT) is a monomeric glycosylated protein; it is the potent inhibitor of a whole range of serine proteases and protects tissues against their destructive effects. The human plasma-derived AAT, which is currently used to augment the AAT level in patients, is limited due to high cost and source limitation. Recombinant production of AAT can be considered as a potential alternative.Objectives: This study aims to develop and optimize a new chemically defi ned medium based on an elemental analysis of the yeast Pichia pastoris for an effi cient culture of the recombinant yeast-producing secretory AAT.Materials and Methods: An elemental analysis of Carbon (C), Hydrogen (H), Nitrogen (N), Sulfur (S); CHNS in its

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دروس نیمسال جاری

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